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c terminal flag tag  (Sino Biological)


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    Sino Biological c terminal flag tag
    C Terminal Flag Tag, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/c+terminal+flag+tag/Human+CDK6+Gene+ORF+cDNA+clone+expression+plasmid%2C+C-Flag+tag/pm41534431-52-9-16
    Average 94 stars, based on 3 article reviews
    c terminal flag tag - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Expressing:

    Article Title: Exploring the characteristics of the SKP2-CDK6 axis in pancreatic cancer cell metastasis and its clinical significance.
    Article Snippet: To investigate the expression of CDK6 and SKP2 in PDAC tissues, we obtained commercial paraffinembedded tissue array slides containing 80 PDAC tissues (Catalog No. PA721a and PA621a, US Biomax, Derwood, MD, USA). .. The expression vector for human CDK6, tagged with a C-terminal Flag tag (pCMV3CDK6-Flag), was purchased from Sino Biological Inc. (Wayne, PA, USA; Cat. No. HG11779-CF). ..

    Article Title: Oligomeric cystatin C supports the immunosuppressive activity of myeloid cells through interaction with inhibitory receptors.
    Article Snippet: The virus-containing supernatant was removed after another 48 h, and monoclonal B16-F10 or THP-1 cells were selected with 5 μg/mL puromycin (Sigma, cat. no. P7255). .. For the CST3-overexpressing cell lines, CST3 cDNA was cloned from commercial plasmids (Sino Biological, cat. no. HG10439-G) with a C-terminal FLAG tag and inserted into the pLVX lentiviral expression vector under the control of the Ef1α promoter. ..

    Article Title: Unveiling the Role of TMPRSS2 in the Proteolytic Activation of Pandemic and Zoonotic Influenza Viruses and Coronaviruses in Human Airway Cells.
    Article Snippet: .. The expression vectors coding for SARS- or MERS-CoV S with a C-terminal FLAG-tag, pCMV3-MERS-Spike-cFLAG (VG40069-CF) and pCMV3-SARS-Spike-cFLAG (VG40150-CF), as well as a vector encoding human dipeptidyl peptidase 4 (DPP4) with a C-terminal HA-tag, pCMV3-hDPP4-cHA (HG10688-CY), were purchased from Sino Biological, Houston, TX, USA. .. The pcDNA3.1(+) plasmid encoding human angiotensin-converting enzyme 2 (ACE2) with a C-terminal HA-tag was commercially obtained from GeneART (ThermoFisher).

    Article Title: Oligomeric cystatin C supports the immunosuppressive activity of myeloid cells through interaction with inhibitory receptors
    Article Snippet: The virus-containing supernatant was removed after another 48 h, and monoclonal B16-F10 or THP-1 cells were selected with 5 μg/mL puromycin (Sigma, cat. no. P7255). .. For the CST3 -overexpressing cell lines, CST3 cDNA was cloned from commercial plasmids (Sino Biological, cat. no. HG10439-G) with a C-terminal FLAG tag and inserted into the pLVX lentiviral expression vector under the control of the Ef1α promoter. ..

    Article Title: Unveiling the Role of TMPRSS2 in the Proteolytic Activation of Pandemic and Zoonotic Influenza Viruses and Coronaviruses in Human Airway Cells
    Article Snippet: .. The expression vectors coding for SARS- or MERS-CoV S with a C-terminal FLAG-tag, pCMV3-MERS-Spike-cFLAG (VG40069-CF) and pCMV3-SARS-Spike-cFLAG (VG40150-CF), as well as a vector encoding human dipeptidyl peptidase 4 (DPP4) with a C-terminal HA-tag, pCMV3-hDPP4-cHA (HG10688-CY), were purchased from Sino Biological, Houston, TX, USA. .. The pcDNA3.1(+) plasmid encoding human angiotensin-converting enzyme 2 (ACE2) with a C-terminal HA-tag was commercially obtained from GeneART (ThermoFisher).

    Article Title: Heme drives cardiac endothelial senescence in sepsis via STING activation
    Article Snippet: .. To increase the expression of STING and TBK1 in HUVECs, cells were seeded at ∼60% confluence and transfected with either the human STING1 ORF cDNA expression plasmid containing a C-terminal HA tag or the human TBK1 ORF mammalian expression plasmid with a C-terminal Flag tag (Sino Biological, Cat# HG29810-CY and HG11023-CF, respectively). .. Transfection was performed using polyethylenimine (PEI, linear, MW 25,000, Polysciences, Cat# 23966) at a DNA:PEI ratio of 1:3.

    Plasmid Preparation:

    Article Title: Exploring the characteristics of the SKP2-CDK6 axis in pancreatic cancer cell metastasis and its clinical significance.
    Article Snippet: To investigate the expression of CDK6 and SKP2 in PDAC tissues, we obtained commercial paraffinembedded tissue array slides containing 80 PDAC tissues (Catalog No. PA721a and PA621a, US Biomax, Derwood, MD, USA). .. The expression vector for human CDK6, tagged with a C-terminal Flag tag (pCMV3CDK6-Flag), was purchased from Sino Biological Inc. (Wayne, PA, USA; Cat. No. HG11779-CF). ..

    Article Title: Oligomeric cystatin C supports the immunosuppressive activity of myeloid cells through interaction with inhibitory receptors.
    Article Snippet: The virus-containing supernatant was removed after another 48 h, and monoclonal B16-F10 or THP-1 cells were selected with 5 μg/mL puromycin (Sigma, cat. no. P7255). .. For the CST3-overexpressing cell lines, CST3 cDNA was cloned from commercial plasmids (Sino Biological, cat. no. HG10439-G) with a C-terminal FLAG tag and inserted into the pLVX lentiviral expression vector under the control of the Ef1α promoter. ..

    Article Title: Unveiling the Role of TMPRSS2 in the Proteolytic Activation of Pandemic and Zoonotic Influenza Viruses and Coronaviruses in Human Airway Cells.
    Article Snippet: .. The expression vectors coding for SARS- or MERS-CoV S with a C-terminal FLAG-tag, pCMV3-MERS-Spike-cFLAG (VG40069-CF) and pCMV3-SARS-Spike-cFLAG (VG40150-CF), as well as a vector encoding human dipeptidyl peptidase 4 (DPP4) with a C-terminal HA-tag, pCMV3-hDPP4-cHA (HG10688-CY), were purchased from Sino Biological, Houston, TX, USA. .. The pcDNA3.1(+) plasmid encoding human angiotensin-converting enzyme 2 (ACE2) with a C-terminal HA-tag was commercially obtained from GeneART (ThermoFisher).

    Article Title: Oligomeric cystatin C supports the immunosuppressive activity of myeloid cells through interaction with inhibitory receptors
    Article Snippet: The virus-containing supernatant was removed after another 48 h, and monoclonal B16-F10 or THP-1 cells were selected with 5 μg/mL puromycin (Sigma, cat. no. P7255). .. For the CST3 -overexpressing cell lines, CST3 cDNA was cloned from commercial plasmids (Sino Biological, cat. no. HG10439-G) with a C-terminal FLAG tag and inserted into the pLVX lentiviral expression vector under the control of the Ef1α promoter. ..

    Article Title: Unveiling the Role of TMPRSS2 in the Proteolytic Activation of Pandemic and Zoonotic Influenza Viruses and Coronaviruses in Human Airway Cells
    Article Snippet: .. The expression vectors coding for SARS- or MERS-CoV S with a C-terminal FLAG-tag, pCMV3-MERS-Spike-cFLAG (VG40069-CF) and pCMV3-SARS-Spike-cFLAG (VG40150-CF), as well as a vector encoding human dipeptidyl peptidase 4 (DPP4) with a C-terminal HA-tag, pCMV3-hDPP4-cHA (HG10688-CY), were purchased from Sino Biological, Houston, TX, USA. .. The pcDNA3.1(+) plasmid encoding human angiotensin-converting enzyme 2 (ACE2) with a C-terminal HA-tag was commercially obtained from GeneART (ThermoFisher).

    Article Title: Inducing production of full-length progranulin (GRN) from nucleotides including mutations containing a premature stop codon (PTC)
    Article Snippet: .. The WT progranulin plasmid with a C-terminal FLAG tag (pC-Flag-PGRN-WT) was purchased from Sino Biological Inc. (Cat. HG10826-CF). .. Three nonsense mutations, Q125X, R229X and R493X, were generated using Q5 site-directed mutagenesis kit (New England Biolabs Inc., Cat. E0554S) to introduce a single nucleotide substitution in the WT progranulin gene (FIG. 1A).

    Article Title: Heme drives cardiac endothelial senescence in sepsis via STING activation
    Article Snippet: .. To increase the expression of STING and TBK1 in HUVECs, cells were seeded at ∼60% confluence and transfected with either the human STING1 ORF cDNA expression plasmid containing a C-terminal HA tag or the human TBK1 ORF mammalian expression plasmid with a C-terminal Flag tag (Sino Biological, Cat# HG29810-CY and HG11023-CF, respectively). .. Transfection was performed using polyethylenimine (PEI, linear, MW 25,000, Polysciences, Cat# 23966) at a DNA:PEI ratio of 1:3.

    FLAG-tag:

    Article Title: Exploring the characteristics of the SKP2-CDK6 axis in pancreatic cancer cell metastasis and its clinical significance.
    Article Snippet: To investigate the expression of CDK6 and SKP2 in PDAC tissues, we obtained commercial paraffinembedded tissue array slides containing 80 PDAC tissues (Catalog No. PA721a and PA621a, US Biomax, Derwood, MD, USA). .. The expression vector for human CDK6, tagged with a C-terminal Flag tag (pCMV3CDK6-Flag), was purchased from Sino Biological Inc. (Wayne, PA, USA; Cat. No. HG11779-CF). ..

    Article Title: Oligomeric cystatin C supports the immunosuppressive activity of myeloid cells through interaction with inhibitory receptors.
    Article Snippet: The virus-containing supernatant was removed after another 48 h, and monoclonal B16-F10 or THP-1 cells were selected with 5 μg/mL puromycin (Sigma, cat. no. P7255). .. For the CST3-overexpressing cell lines, CST3 cDNA was cloned from commercial plasmids (Sino Biological, cat. no. HG10439-G) with a C-terminal FLAG tag and inserted into the pLVX lentiviral expression vector under the control of the Ef1α promoter. ..

    Article Title: Oligomeric cystatin C supports the immunosuppressive activity of myeloid cells through interaction with inhibitory receptors
    Article Snippet: The virus-containing supernatant was removed after another 48 h, and monoclonal B16-F10 or THP-1 cells were selected with 5 μg/mL puromycin (Sigma, cat. no. P7255). .. For the CST3 -overexpressing cell lines, CST3 cDNA was cloned from commercial plasmids (Sino Biological, cat. no. HG10439-G) with a C-terminal FLAG tag and inserted into the pLVX lentiviral expression vector under the control of the Ef1α promoter. ..

    Article Title: Inducing production of full-length progranulin (GRN) from nucleotides including mutations containing a premature stop codon (PTC)
    Article Snippet: .. The WT progranulin plasmid with a C-terminal FLAG tag (pC-Flag-PGRN-WT) was purchased from Sino Biological Inc. (Cat. HG10826-CF). .. Three nonsense mutations, Q125X, R229X and R493X, were generated using Q5 site-directed mutagenesis kit (New England Biolabs Inc., Cat. E0554S) to introduce a single nucleotide substitution in the WT progranulin gene (FIG. 1A).

    Article Title: Heme drives cardiac endothelial senescence in sepsis via STING activation
    Article Snippet: .. To increase the expression of STING and TBK1 in HUVECs, cells were seeded at ∼60% confluence and transfected with either the human STING1 ORF cDNA expression plasmid containing a C-terminal HA tag or the human TBK1 ORF mammalian expression plasmid with a C-terminal Flag tag (Sino Biological, Cat# HG29810-CY and HG11023-CF, respectively). .. Transfection was performed using polyethylenimine (PEI, linear, MW 25,000, Polysciences, Cat# 23966) at a DNA:PEI ratio of 1:3.

    Article Title: miR-573 rescues endothelial dysfunction during dengue infection under PPARγ regulation
    Article Snippet: DENV2 structural proteins were cloned into expression vector from Promega in frame with a C-terminal Flag tag. .. PPARγ cDNA clone with the C-terminal Flag tag was purchased from (HG12019-CF, Sino biological). .. The 3’UTR luciferase constructs were designed using the pmiRGLO Dual luciferase miRNA target expression vector.

    Clone Assay:

    Article Title: Oligomeric cystatin C supports the immunosuppressive activity of myeloid cells through interaction with inhibitory receptors.
    Article Snippet: The virus-containing supernatant was removed after another 48 h, and monoclonal B16-F10 or THP-1 cells were selected with 5 μg/mL puromycin (Sigma, cat. no. P7255). .. For the CST3-overexpressing cell lines, CST3 cDNA was cloned from commercial plasmids (Sino Biological, cat. no. HG10439-G) with a C-terminal FLAG tag and inserted into the pLVX lentiviral expression vector under the control of the Ef1α promoter. ..

    Article Title: Oligomeric cystatin C supports the immunosuppressive activity of myeloid cells through interaction with inhibitory receptors
    Article Snippet: The virus-containing supernatant was removed after another 48 h, and monoclonal B16-F10 or THP-1 cells were selected with 5 μg/mL puromycin (Sigma, cat. no. P7255). .. For the CST3 -overexpressing cell lines, CST3 cDNA was cloned from commercial plasmids (Sino Biological, cat. no. HG10439-G) with a C-terminal FLAG tag and inserted into the pLVX lentiviral expression vector under the control of the Ef1α promoter. ..

    Control:

    Article Title: Oligomeric cystatin C supports the immunosuppressive activity of myeloid cells through interaction with inhibitory receptors.
    Article Snippet: The virus-containing supernatant was removed after another 48 h, and monoclonal B16-F10 or THP-1 cells were selected with 5 μg/mL puromycin (Sigma, cat. no. P7255). .. For the CST3-overexpressing cell lines, CST3 cDNA was cloned from commercial plasmids (Sino Biological, cat. no. HG10439-G) with a C-terminal FLAG tag and inserted into the pLVX lentiviral expression vector under the control of the Ef1α promoter. ..

    Article Title: Oligomeric cystatin C supports the immunosuppressive activity of myeloid cells through interaction with inhibitory receptors
    Article Snippet: The virus-containing supernatant was removed after another 48 h, and monoclonal B16-F10 or THP-1 cells were selected with 5 μg/mL puromycin (Sigma, cat. no. P7255). .. For the CST3 -overexpressing cell lines, CST3 cDNA was cloned from commercial plasmids (Sino Biological, cat. no. HG10439-G) with a C-terminal FLAG tag and inserted into the pLVX lentiviral expression vector under the control of the Ef1α promoter. ..

    Transfection:

    Article Title: Heme drives cardiac endothelial senescence in sepsis via STING activation
    Article Snippet: .. To increase the expression of STING and TBK1 in HUVECs, cells were seeded at ∼60% confluence and transfected with either the human STING1 ORF cDNA expression plasmid containing a C-terminal HA tag or the human TBK1 ORF mammalian expression plasmid with a C-terminal Flag tag (Sino Biological, Cat# HG29810-CY and HG11023-CF, respectively). .. Transfection was performed using polyethylenimine (PEI, linear, MW 25,000, Polysciences, Cat# 23966) at a DNA:PEI ratio of 1:3.



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